BIOTAQ and ACROBiosystems joining
forces to become a world leader in bulk protein and monoclonal antibody
production.......
500+ active rProtein/150+ MAb CRO projects experience
3 g/L MAb production cell line and scale up process development
200g/batch MAb manufacturing for clients
10 CRO projects from cell line development, PD to pre-clinical production
Affordable & Flexible Gene to Protein
Services /MAb Only in 3-4 Weeks / World Top Quality
Contract Service
World-leading mammalian/ E.coli expression
and purification platforms:
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Risk free business model
with ultra fast small scale evaluation....
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One stop services
Integration from gene synthesizing to end-point product delivery
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High flexibility for mg to g
production of bio-reagent or pre-clinical materials starting from
gene,cell line or culture broth
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Premier product quality
assurance from experienced scientists with premier process
understanding , in-process control and QC systems
Our Service
Offering Various Platform Options:
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Recombinant protein/MAb
production by 293/CHO transient transfection
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Recombinant protein/MAb
production by stable CHO cell line
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MAb production by hybridoma
cell lines
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Recombinant protein
production by E. coli
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Recombinant protein
production by insect cells(under development)
Integrated
Platform Overview Our Strength, Your Wise Choice:
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In-house developed
proprietary mammalian expression systems with ultra high expression level
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In-house developed
proprietary chemically defined medium/feeds, rapid medium optimization
platform
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State-of-Art process
development technologies for large scale cell culture and downstream
processing
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Well established QC systems
Integrated
Platform Molecular Biology:
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Time saving codon
optimization and gene synthesizing services
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One stop molecular services
Integration
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In-house developed codon
optimization system
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In-house Gene synthesizing
and sequencing
Proprietary
developed best expression vectors
rProtein/MAb expression special optimization
2-5 times expression level higher than commercial vectors
In-house proprietary developed HEK293 host
cell line / 10 times expression level higher than commercial host cell lines
Unique screening strategy in stable
cell line development
Fluorescence based screening approach saves >2 months and increase 5 times
throughput
Integrated Platform Cell Culture and
Fermentation:
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In-house developed CD medium
and feeds for transient expression
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Chemically defined 293/CHO
transfection and expression medium support high transfection efficiency up
to 80% in suspension culture
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Robust medium
formulations support both transfection and expression, medium exchange is
not reqiured at any time during process
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Chemically defined feeds
enable process duration longer (>10days)with up to 75% cell viability.
Process development with scalability for
stable cell line project:
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Rapid medium/feeds
optimization integrated to early stage of cell line development from clone
screening (chemically defined and animal origin free)
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DoE based statistical
approaches
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Scale down model study
ensure scalability of process
Integrated Platform /
Purification/Formulation/Analytical:
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More than 10 years’
experience in protein technologies, such as chromatography, filtration,
refolding, lyo, formulation, etc
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HTPD and DoE technologies
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Generic therapeutic MAb
purification platform with >80% recovery
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AC/IEX/HIC/RPC/GF
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CFF/NFF, both
microfiltration and ultrafiltration
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Refolding,
dilution/dialysis/hollow fiber/on-column refolding, etc
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Process scale up and
validation
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Formulation screening
and stability test
E.
coli Based Protein Production
You provide:
Gene sequences or cDNA Clone
We will do:
Gene synthesizing and codon optimization
Expression vector construction
In house developed medium fermentation
Purification process for tag or non-tag recombinant protein
Purification and refolding of inclusion body
You will get:
mg-g scale purified proteins
CoA / CoQ
Project Report
turnaround 3 to 4 weeks
CHO/293 cells Based Transient
Expression
You provide:
Gene sequences or cDNA clone
We will do:
Codon optimization and gene synthesis
293 as expression host or CHO for suspension transfection and
expression.
Two or three-step purification process for MAb, fusion proteins and
non-tag rprotein
You will get:
mg-g scale purified proteins or MAb
CoA / CoQ
Project report
in 4 to 6 weeks
Stable CHO Cell Line Generation and
Protein Production
You provide
Gene sequences or cDNA
We will do:
Codon optimization and gene synthesis
Expression vector construction, transfection and clone selection using CHO
cell.
Process development and production
Two or three-step purification process for MAb, fusion proteins and non-tag
rprotein
you will get:
mg-100g scale Purified proteins or MAb
CoA / CoQ
Project report
in 4 to 7 weeks
Serum Free, Cell Culture Process
Production for Various Cell Line In Large Scale, and Purification If
Applicable
You provide:
Cell line
We will do:
CHO, 293,NS0,Hybridoma,Per.C6, Sf21, Sf9 cells adaptation to serum free medium
and banking
Serum free medium is must, most culture process use protein free or
chemically defined medium;
Culture volume range from 100mL to 4X 10L
Purification process/formulation/analytical development
you will get:
Purified proteins if applicable
Key process information
CoA / CoQ
Project report
We also have these proteins in stock:
Human PCSK9
Human ACVR2B
Mouse RSPO1
Human DKK3
Human FGFR1
Human TNFa
Human aFGF
Hot products
Human DKK1
Human EGFR
Human HER2
Human BMP2
Human bFGF
Human NGF
Human VEGF165
Human TGFB1
Recommended products:
Super Protein A
Protein G
Protein L
Human KDR
Human FGFR4
Human EPHB4
Human IGF-1R
gp120(JR-FL)......
Please contact me for more details at nchen@biotaq.com