Polyacrylamide Coated Capillary Regeneration Buffer(PAMC-RB)
The most widely used capillary coating method is
linear polyacrylamide coating, which was first introduced by Hjerten, it is
via silanization first using bifunctional silane reagent (r-methacryloxypropyltrimethoxysilane).
The molecule, which is
attached to the surface via silanization, contains double bond, by which this group will be co-polymerized with acrylamide
to form a linear polyacrylamide coating layer, such polar coating minimizes protein adsorption, and effectively suppresses
electroosmotic flow (EOF) by neutralizing the negative charges on the capillary wall, and has been widely used for protein and
DNA capillary electrophoresis separations. However polyacrylamide coating goes unstable after 10 to 20 injections, evidenced
by the shift in migration time.
Proprietary Polyacrylamide Coated Capillary Regeneration Buffer (PAMC-RB) is designed to regenerate your old polyacrylamide
coated capillary after it goes unstable or bad. The regeneration procedure is very simple to follow, and can be easily programmed
into any capillary electrophoresis systems. The regenerated capillary shows the excellent stability.
Another alternative, is to use our INSTCoating Solution (BT200-01)
as a replacement for polyacrylamide coating, doing
so will just make you drop the whole bind silane thing (plus, it smells, it is
toxic, you need to operate it in the hood....who
likes this kind of stuff??), ask us for direction.
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Polyacrylamide Coated Capillary Regeneration Buffer(PAMC-RB)
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Polyacrylamide Coated Capillary Regeneration Buffer (PAMC-RB) (1ml),
$150
RNA isolation RNA extraction RNA isolation RNA
extraction RNA isolation RNA extraction RNA isolation RNA extraction RNA
isolation RNA extraction RNA isolation RNA extraction
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